For the Prega News Pregnancy Test Kit, the most accurate results are typically obtained using morning urine, as it contains higher levels of the pregnancy hormone hCG (human chorionic gonadotropin). However, you can still test in the afternoon, but the results may be less reliable if the urine is more diluted. It’s best to test with your first morning urine for the most accurate results. If testing in the afternoon, try to avoid drinking large amounts of fluids before testing, as this can dilute the urine.